Timopoetina
As timopoetinas, timopoietinas ou isoformas beta/gamma do polipéptido 2 asociado ás laminas [1] son unhas proteínas codificadas polo xene TMPO nos humanos.[2][3]
A timopoetina foi illada inicialmente do timo bovino e déuselle primeiro o nome de timina, que foi cambiado a timopoetina, para non confundila coa base nitroxenada timina [4].
Á timopoetina atribúeselle unha acción hormonal, que induce a diferenciación dos timocitos a partir das súas células precursoras [5].
O xene da timopoetina (TMPO) codifica tres ARNm, resultado dun splicing alternativo, que orixinan tres proteínas, de 75 kDa (alfa), 51 kDa (beta) e 39 kDa (gamma), que son expresadas en todas as células. O xene TMPO humano atópase na banda 12q22 do cromosoma 22 e consta de oito exóns. O TMPO alfa está presente e difusamente expresado no núcleo celular, e os TMPO beta e gamma están localizados na membrana nuclear. O TMPO beta é un homólogo humano da proteína murina LAP2 (polipéptido 2 asociado á lamina). O LAP2 desempeña un papel na regulación da arquitectura nuclear ao unir a proteína lamina B1 cos cromosomas e intervir na formación da lámina nuclear. Esta interacción está regulada por fosforilación durante a mitose. Dada a localización nuclear das tres isoformas de TMPO, é improbable que estas proteínas xoguen un papel na indución do CD90 no timo, como se suxerira.
Interaccións[editar | editar a fonte]
A timopoetina interacciona co factor barreira de autointegración 1,[6] AKAP8L,[7][8] LMNB1[9][10] e co LMNA.[11][12]
Notas[editar | editar a fonte]
- ↑ "TMPO thymopoietin". Arquivado dende o orixinal o 07 de marzo de 2016. Consultado o 29 de xaneiro de 2012.
- ↑ Harris CA, Andryuk PJ, Cline S, Chan HK, Natarajan A, Siekierka JJ, Goldstein G (1994). "Three distinct human thymopoietins are derived from alternatively spliced mRNAs". Proc Natl Acad Sci U S A 91 (14): 6283–7. PMC 44185. PMID 7517549. doi:10.1073/pnas.91.14.6283.
- ↑ "Entrez Gene: TMPO thymopoietin".
- ↑ Gideon Goldstein. The isolation of thymopoietin (thymin). Annals of the New York Academy of Sciences. Volume 249, Thymus Factors in Immunity pages 177–185, February 1975 [1]
- ↑ J J Twomey, G Goldstein, V M Lewis, P M Bealmear, R A Good. Bioassay determinations of thymopoietin and thymic hormone levels in human plasma. Proceedings of the National Academy of Science of USA. June 1, 1977 vol. 74 no. 6 2541-2545. [2]
- ↑ Furukawa, K (1999). "LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction". J. Cell. Sci. (ENGLAND) 112 (15): 2485–92. ISSN 0021-9533. PMID 10393804.
- ↑ Martins, S B; Eide T, Steen R L, Jahnsen T, Skålhegg B S, Collas P (2000). "HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics". J. Cell. Sci. (ENGLAND) 113 (21): 3703–13. ISSN 0021-9533. PMID 11034899.
- ↑ Martins, Sandra; Eikvar Sissel, Furukawa Kazuhiro, Collas Philippe (2003). "HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication". J. Cell Biol. (United States) 160 (2): 177–88. ISSN 0021-9525. PMC 2172640. PMID 12538639. doi:10.1083/jcb.200210026.
- ↑ Furukawa, K; Kondo T (1998). "Identification of the lamina-associated-polypeptide-2-binding domain of B-type lamin". Eur. J. Biochem. (GERMANY) 251 (3): 729–33. ISSN 0014-2956. PMID 9490046. doi:10.1046/j.1432-1327.1998.2510729.x.
- ↑ Foisner, R; Gerace L (1993). "Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation". Cell (UNITED STATES) 73 (7): 1267–79. ISSN 0092-8674. PMID 8324822. doi:10.1016/0092-8674(93)90355-T.
- ↑ Markiewicz, Ewa; Dechat Thomas, Foisner Roland, Quinlan Roy A, Hutchison Christopher J (2002). "Lamin A/C binding protein LAP2alpha is required for nuclear anchorage of retinoblastoma protein". Mol. Biol. Cell (United States) 13 (12): 4401–13. ISSN 1059-1524. PMC 138642. PMID 12475961. doi:10.1091/mbc.E02-07-0450.
- ↑ Dechat, T; Korbei B, Vaughan O A, Vlcek S, Hutchison C J, Foisner R (2000). "Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins". J. Cell. Sci. (ENGLAND) 113 (19): 3473–84. ISSN 0021-9533. PMID 10984438.
Véxase tamén[editar | editar a fonte]
Bibliografía[editar | editar a fonte]
- Harris CA, Andryuk PJ, Cline SW, Mathew S, Siekierka JJ, Goldstein G (1995). "Structure and mapping of the human thymopoietin (TMPO) gene and relationship of human TMPO beta to rat lamin-associated polypeptide 2". Genomics 28 (2): 198–205. PMID 8530026. doi:10.1006/geno.1995.1131.
- Dechat T, Vlcek S, Foisner R (2000). "Review: lamina-associated polypeptide 2 isoforms and related proteins in cell cycle-dependent nuclear structure dynamics.". J. Struct. Biol. 129 (2-3): 335–45. PMID 10806084. doi:10.1006/jsbi.2000.4212.
- Twomey JJ, Goldstein G, Lewis VM; et al. (1977). "Bioassay determinations of thymopoietin and thymic hormone levels in human plasma.". Proc. Natl. Acad. Sci. U.S.A. 74 (6): 2541–5. PMC 432209. PMID 302007. doi:10.1073/pnas.74.6.2541.
- Heavner GA, Audhya T, Goldstein G (1990). "Peptide analogs of thymopentin distinguish distinct thymopoietin receptor specificities on two human T cell lines.". Regul. Pept. 27 (2): 257–62. PMID 2158125. doi:10.1016/0167-0115(90)90044-W.
- Audhya T, Schlesinger DH, Goldstein G (1987). "Isolation and complete amino acid sequence of human thymopoietin and splenin.". Proc. Natl. Acad. Sci. U.S.A. 84 (11): 3545–9. PMC 304911. PMID 3473468. doi:10.1073/pnas.84.11.3545.
- Fuccello A, Audhya T, Talle MA, Goldstein G (1984). "Immunoassay for bovine serum thymopoietin: discrimination from splenin by monoclonal antibodies.". Arch. Biochem. Biophys. 228 (1): 292–8. PMID 6364989. doi:10.1016/0003-9861(84)90070-5.
- Hara H, Hayashi K, Ohta K; et al. (1995). "A new thymopoietin precursor gene from human thymus.". Biochem. Mol. Biol. Int. 34 (5): 927–33. PMID 7703909.
- Goldstein G, Schlesinger DH, Audhya T (1994). "Isolation and complete amino acid sequence of human thymopoietin and splenin.". Proc. Natl. Acad. Sci. U.S.A. 91 (13): 6249. PMC 44176. PMID 8016147. doi:10.1073/pnas.91.13.6249.
- Harris CA, Andryuk PJ, Cline SW; et al. (1996). "Structure and mapping of the human thymopoietin (TMPO) gene and relationship of human TMPO beta to rat lamin-associated polypeptide 2.". Genomics 28 (2): 198–205. PMID 8530026. doi:10.1006/geno.1995.1131.
- Andersson B, Wentland MA, Ricafrente JY; et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. PMID 8619474. doi:10.1006/abio.1996.0138.
- Berger R, Theodor L, Shoham J; et al. (1996). "The characterization and localization of the mouse thymopoietin/lamina-associated polypeptide 2 gene and its alternatively spliced products.". Genome Res. 6 (5): 361–70. PMID 8743987. doi:10.1101/gr.6.5.361.
- Yu W, Andersson B, Worley KC; et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. PMC 139146. PMID 9110174. doi:10.1101/gr.7.4.353.
- Furukawa K, Fritze CE, Gerace L (1998). "The major nuclear envelope targeting domain of LAP2 coincides with its lamin binding region but is distinct from its chromatin interaction domain.". J. Biol. Chem. 273 (7): 4213–9. PMID 9461618. doi:10.1074/jbc.273.7.4213.
- Furukawa K, Kondo T (1998). "Identification of the lamina-associated-polypeptide-2-binding domain of B-type lamin.". Eur. J. Biochem. 251 (3): 729–33. PMID 9490046. doi:10.1046/j.1432-1327.1998.2510729.x.
- Dechat T, Gotzmann J, Stockinger A; et al. (1998). "Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics.". EMBO J. 17 (16): 4887–902. PMC 1170818. PMID 9707448. doi:10.1093/emboj/17.16.4887.
- Furukawa K (1999). "LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction.". J. Cell. Sci. 112 (15): 2485–92. PMID 10393804.
- Weber PJ, Eckhard CP, Gonser S; et al. (1999). "On the role of thymopoietins in cell proliferation. Immunochemical evidence for new members of the human thymopoietin family.". Biol. Chem. 380 (6): 653–60. PMID 10430029. doi:10.1515/BC.1999.081.
- Dechat T, Korbei B, Vaughan OA; et al. (2000). "Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins.". J. Cell. Sci. 113 (19): 3473–84. PMID 10984438.
- Martins SB, Eide T, Steen RL; et al. (2001). "HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics.". J. Cell. Sci. 113 (21): 3703–13. PMID 11034899.
Ligazóns externas[editar | editar a fonte]
- MeshName - Thymopoietins [3]
