Coilina

A coilina é unha proteína que nos humanos está codificada polo xene COIL.^[1]^[2]

A proteína coilina é un dos principais compoñentes dos corpos de Cajal. A coilina recibe o seu nome da forma enrolada que teñen os corpos de Cajal onde se atopan (do inglés coil, enrolar). Foi identificada inicialmente utilizando soro autoinmune humano. Os corpos de Cajal son suborgánulos celulares que se encontran no núcleo en número variable e con diversa composición, que están implicados na modificación postranscricional de ARNs nucleares pequenos e ARN nucleolares pequenos. Ademais do seu papel estrutural, a coilina actúa como un pegamento que conecta os corpos de Cajal co nucléolo. O extremo N-terminal da coilina dirixe a súa propia oligomerización e o extremo C-terminal inflúe no número de corpos nucleares que se ensamblan por célula. A metilación e fosforilación diferencial da coilina inflúe probablemente na súa localización entre os corpos nucleares e a composición e ensamblaxe dos corpos de Cajal. O xene da coilina ten pseudoxenes nos cromosomas 4 e 14.^[2]

Para estudar os corpos de Cajal, pode facerse que a coilina se combine coa proteína fluorescente verde (GFP) para formar a proteína híbrida coilina-GFP. Esta proteína híbrida pode despois utilizarse para localizas os corpos de Cajal en exame microscópico, que xeralmente están preto do nucléolo do célula. Outras proteínas que forman parte dos corpos de Cajal son as ribonucleoproteínas nucleares pequenas (snRNP) e as ribonucleoproteínas nucleolares pequenas (snoRNP).

Interaccións[editar | editar a fonte]

A coilina presenta interaccións coa SMN1,^[3]^[4] a SNRPB,^[4] a fosfoproteína nucleolar p130^[5] e a ataxina 1.^[6]^[7]

Notas[editar | editar a fonte]

↑ Chan EK, Takano S, Andrade LE, Hamel JC, Matera AG (Dec 1994). "Structure, expression and chromosomal localization of human p80-coilin gene". Nucleic Acids Res 22 (21): 4462–9. PMC 308480. PMID 7971277. doi:10.1093/nar/22.21.4462.
↑ ^2,0 ^2,1 "Entrez Gene: COIL coilin".
↑ Hebert, Michael D; Shpargel Karl B, Ospina Jason K, Tucker Karen E, Matera A Gregory (Sep 2002). "Coilin methylation regulates nuclear body formation". Dev. Cell (United States) 3 (3): 329–37. ISSN 1534-5807. PMID 12361597. doi:10.1016/S1534-5807(02)00222-8.
↑ ^4,0 ^4,1 Hebert, M D; Szymczyk P W, Shpargel K B, Matera A G (Oct 2001). "Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein". Genes Dev. (United States) 15 (20): 2720–9. ISSN 0890-9369. PMC 312817. PMID 11641277. doi:10.1101/gad.908401.
↑ Isaac, C; Yang Y, Meier U T (Jul 1998). "Nopp140 functions as a molecular link between the nucleolus and the coiled bodies". J. Cell Biol. (UNITED STATES) 142 (2): 319–29. ISSN 0021-9525. PMC 2133063. PMID 9679133. doi:10.1083/jcb.142.2.319.
↑ Hong, Sunghoi; Ka Sojeong, Kim Sungjo, Park Yongjae, Kang Seongman (May 2003). "p80 coilin, a coiled body-specific protein, interacts with ataxin-1, the SCA1 gene product". Biochim. Biophys. Acta (Netherlands) 1638 (1): 35–42. ISSN 0006-3002. PMID 12757932. doi:10.1016/S0925-4439(03)00038-3.
↑ Hong, Sunghoi; Lee Soyeon, Cho Ssang-Goo, Kang Seongman (Jun 2008). "UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1". Biochem. Biophys. Res. Commun. (United States) 371 (2): 256–60. PMID 18439907. doi:10.1016/j.bbrc.2008.04.066.

Véxase tamén[editar | editar a fonte]

Bibliografía[editar | editar a fonte]

Ogg SC, Lamond AI (October 14, 2002). "Cajal bodies and coilin — moving towards function". J. Cell Biol. 159 (1): 17–21. PMC 2173504. PMID 12379800. doi:10.1083/jcb.200206111.
Andrade LE; Chan EK; Raska I; et al. (1991). "Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin". J. Exp. Med. 173 (6): 1407–19. PMC 2190846. PMID 2033369. doi:10.1084/jem.173.6.1407.
Bohmann K, Ferreira JA, Lamond AI (1996). "Mutational analysis of p80 coilin indicates a functional interaction between coiled bodies and the nucleolus". J. Cell Biol. 131 (4): 817–31. PMC 2200013. PMID 7490287. doi:10.1083/jcb.131.4.817.
Carmo-Fonseca M, Ferreira J, Lamond AI (1993). "Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis--evidence that the coiled body is a kinetic nuclear structure". J. Cell Biol. 120 (4): 841–52. PMC 2200076. PMID 7679389. doi:10.1083/jcb.120.4.841.
Robertson NG; Khetarpal U; Gutiérrez-Espeleta GA; et al. (1995). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics 23 (1): 42–50. PMID 7829101. doi:10.1006/geno.1994.1457.
Kusuda J; Tanuma R; Gotoh E; et al. (1995). "Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14". Hum. Genet. 95 (2): 233–4. PMID 7860074. doi:10.1007/BF00209411.
Lyon CE, Bohmann K, Sleeman J, Lamond AI (1997). "Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus". Exp. Cell Res. 230 (1): 84–93. PMID 9013710. doi:10.1006/excr.1996.3380.
Parfenov VN; Davis DS; Pochukalina GN; et al. (1998). "Dynamics of distribution of splicing components relative to the transcriptional state of human oocytes from antral follicles". J. Cell. Biochem. 69 (1): 72–80. PMID 9513048. doi:10.1002/(SICI)1097-4644(19980401)69:1<72::AID-JCB8>3.0.CO;2-S.
Pochukalina GN; Davis DS; Kostiuchek DF; et al. (1998). "[Splicing factors in oocyte nuclei from human antral follicles]". Tsitologiia 40 (4): 239–47. PMID 9644758.
Isaac C, Yang Y, Meier UT (1998). "Nopp140 functions as a molecular link between the nucleolus and the coiled bodies". J. Cell Biol. 142 (2): 319–29. PMC 2133063. PMID 9679133. doi:10.1083/jcb.142.2.319.
Liu J; Hebert MD; Ye Y; et al. (2000). "Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies". J. Cell. Sci. 113 (9): 1543–52. PMID 10751146.
Hebert MD, Matera AG (2001). "Self-association of coilin reveals a common theme in nuclear body localization". Mol. Biol. Cell 11 (12): 4159–71. PMC 15064. PMID 11102515.
Young PJ; Le TT; Dunckley M; et al. (2001). "Nuclear gems and Cajal (coiled) bodies in fetal tissues: nucleolar distribution of the spinal muscular atrophy protein, SMN". Exp. Cell Res. 265 (2): 252–61. PMID 11302690. doi:10.1006/excr.2001.5186.
Hebert MD, Szymczyk PW, Shpargel KB, Matera AG (2001). "Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein". Genes Dev. 15 (20): 2720–9. PMC 312817. PMID 11641277. doi:10.1101/gad.908401.
Andersen JS; Lyon CE; Fox AH; et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. PMID 11790298. doi:10.1016/S0960-9822(01)00650-9.
Sleeman JE, Ajuh P, Lamond AI (2002). "snRNP protein expression enhances the formation of Cajal bodies containing p80-coilin and SMN". J. Cell. Sci. 114 (Pt 24): 4407–19. PMID 11792806.
Hebert MD; Shpargel KB; Ospina JK; et al. (2002). "Coilin methylation regulates nuclear body formation". Dev. Cell 3 (3): 329–37. PMID 12361597. doi:10.1016/S1534-5807(02)00222-8.
Strausberg RL; Feingold EA; Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. PMC 139241. PMID 12477932. doi:10.1073/pnas.242603899.
Shpargel KB; Ospina JK; Tucker KE; et al. (2003). "Control of Cajal body number is mediated by the coilin C-terminus". J. Cell. Sci. 116 (Pt 2): 303–12. PMID 12482916. doi:10.1242/jcs.00211.
Sleeman JE; Trinkle-Mulcahy L; Prescott AR; et al. (2004). "Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo". J. Cell. Sci. 116 (Pt 10): 2039–50. PMID 12679382. doi:10.1242/jcs.00400.
Hearst SM; Gilder AS; Negi SS; Davis MD; George EM; Whittom AA; Toyota CG; Husedzinovic A; Gruss OJ (2009). "Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines". J. Cell. Sci. 122 (Pt 11): 1872–81. PMC 2684838. PMID 19435804. doi:10.1242/jcs.044040.

[pmid7971277-1] Chan EK, Takano S, Andrade LE, Hamel JC, Matera AG (Dec 1994). "Structure, expression and chromosomal localization of human p80-coilin gene". Nucleic Acids Res 22 (21): 4462–9. PMC 308480. PMID 7971277. doi:10.1093/nar/22.21.4462.

[entrez-2] 2,0 ^2,1 "Entrez Gene: COIL coilin".

[pmid12361597-3] Hebert, Michael D; Shpargel Karl B, Ospina Jason K, Tucker Karen E, Matera A Gregory (Sep 2002). "Coilin methylation regulates nuclear body formation". Dev. Cell (United States) 3 (3): 329–37. ISSN 1534-5807. PMID 12361597. doi:10.1016/S1534-5807(02)00222-8.

[pmid11641277-4] 4,0 ^4,1 Hebert, M D; Szymczyk P W, Shpargel K B, Matera A G (Oct 2001). "Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein". Genes Dev. (United States) 15 (20): 2720–9. ISSN 0890-9369. PMC 312817. PMID 11641277. doi:10.1101/gad.908401.

[pmid9679133-5] Isaac, C; Yang Y, Meier U T (Jul 1998). "Nopp140 functions as a molecular link between the nucleolus and the coiled bodies". J. Cell Biol. (UNITED STATES) 142 (2): 319–29. ISSN 0021-9525. PMC 2133063. PMID 9679133. doi:10.1083/jcb.142.2.319.

[pmid12757932-6] Hong, Sunghoi; Ka Sojeong, Kim Sungjo, Park Yongjae, Kang Seongman (May 2003). "p80 coilin, a coiled body-specific protein, interacts with ataxin-1, the SCA1 gene product". Biochim. Biophys. Acta (Netherlands) 1638 (1): 35–42. ISSN 0006-3002. PMID 12757932. doi:10.1016/S0925-4439(03)00038-3.

[pmid18439907-7] Hong, Sunghoi; Lee Soyeon, Cho Ssang-Goo, Kang Seongman (Jun 2008). "UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1". Biochem. Biophys. Res. Commun. (United States) 371 (2): 256–60. PMID 18439907. doi:10.1016/j.bbrc.2008.04.066.

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